I. Chemical Glycobiology
II. Imaging the Glycome
Part II: Imaging the Glycome
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A large part of an organism’s complexity is not encoded by its genome but results from post-translational modification. Glycosylation, or the addition of sugar molecules to a protein is an example of such a modification. These sugars, or glycans, are often complex, branched molecules specific to particular cells. Cell surface glycans determine human blood types, allow viral infections and play a key role in tissue inflammation.
Since glycans cannot be labeled with genetically-encoded reporters such as GFP, bioorthoganal reactions have been developed to allow their labeling and imaging. In this lecture, Bertozzi describes the chemistry and imaging methodology used to view glycoproteins in cells and whole organisms.
Carolyn Bertozzi is a professor of Chemistry and Molecular and Cell Biology at the University of California, Berkeley, Director of the Molecular Foundry at the Lawrence Berkeley National Lab and a Howard Hughes Medical Institute Investigator. She received an A.B. degree in chemistry from Harvard and a Ph.D. from UC Berkeley. Her postdoctoral work was done in cellular immunology at UCSF.
The focus of Bertozzi’s research is to understand glycosylation – the normal process by which sugars are added to proteins or lipids. Changes in glycosylation can be associated with illness or infection. Bertozzi’s group has developed chemical tools that allow changes in cell surface sugars to be followed in live animals.
Dr. Bertozzi has won numerous honors and awards including several for excellence in undergraduate teaching. More >>
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Varki, A; Cumming, R.D.; Esko, J.D.; Freeze, H.H.; Hart, G.W.; Etzler, M.E. (2009) Essentials of Glycobiology 2nd ed. Nelson, D.L.; Cox, M.M. (2005) Lehninger Principles of Biochemistry 4th ed. Chapter 7: Carbohydrates and Glycobiology p. 238.
Sletten, E.M.; Bertozzi, C.R.; Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angew Chem Int Ed Engl. 2009, 48, 6974-98.
Chang, P.V.; Prescher, J.A.; Hangauer, M.J.; Bertozzi, C.R.; Imaging cell surface glycans with bioorthogonal chemical reporters. J Am Chem Soc. 2007, 129, 8400-1.
Baskin, J.M.; Prescher, J.A., Laughlin, S.T.; Agard, N.J.; Chang, P.V.; Miller, I.A.; Lo, A.; Codelli, J.A.; Bertozzi, C.R.; Copper-free click chemistry for dynamic in vivo imaging. Proc Natl Acad Sci USA. 2007, 104, 16793-7.