Part III: Protein Kinase Regulation and Localization
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In this lecture, I have given an overview of protein kinase structure and function using cyclic AMP dependent kinase (PKA) as a prototype for this enzyme superfamily. I have demonstrated what we have learned from the overall structural kinome which allows us to compare many protein kinases and also to appreciate how the highly regulated eukaryotic protein kinase has evolved. By comparing many protein kinase structures, we are beginning to elucidate general rules of architecture. In addition, I have attempted to illustrate how PKA is regulated by cAMP and how it is localized to specific macromolecular complexes through scaffold proteins.
Dr. Taylor received her BA in Chemistry from the University of Wisconsin-Madison and her PhD in Physiological Chemistry from Johns Hopkins University. After completing a fellowship at the MRC in Cambridge, she moved to the University of California, San Diego where she soon secured a faculty position in the chemistry department. Shortly after joining UCSD, Taylor began working on PKA and her lab has been investigating the mysteries of its structure and function ever since.
Dr. Taylor has been a Howard Hughes Medical Institute Investigator since 1997. She is a member of the National Academy of Sciences and the American Academy of Arts and Science and she has been awarded numerous prizes for her groundbreaking research.
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Dynamics of cAMP-dependent protein kinase. Johnson DA, Akamine P, Radzio-Andzelm E, Madhusudan M, Taylor SS. Chem Rev. 2001 Aug;101(8):2243-70.
Regulation of protein kinases; controlling activity through activation segment conformation. Nolen B, Taylor S, Ghosh G. Mol Cell. 2004 Sep 10;15(5):661-75.
Active and inactive protein kinases: structural basis for regulation. Johnson LN, Noble ME, Owen DJ. Cell. 1996 Apr 19;85(2):149-58.
A helix scaffold for the assembly of active protein kinases. Kornev AP, Taylor SS, Ten Eyck LF. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14377-82.
Surface comparison of active and inactive protein kinases identifies a conserved activation mechanism. Kornev AP, Haste NM, Taylor SS, Eyck LF. Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17783-8.
The hallmark of AGC kinase functional divergence is its C-terminal tail, a cis-acting regulatory module. Kannan N, Haste N, Taylor SS, Neuwald AF. Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1272-7.