Part III: Mycobacteriophage genomics
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Bacteriophage, viruses that specifically infect bacteria, are, by far, the majority of all biological entities in the biosphere. The viral population, including bacteriophage, is very diverse yet relatively few viral genomes have been sequenced. In this series of lectures, Hatfull argues that viral genomes provide a great source of new genes, potentially with new functions and structures.
In Part 1, Hatfull describes what bacteriophage are, some of their biological properties and how they were discovered. In Part 2, he discusses the organization of bacteriophage genomes and explains how genomic sequence comparison can provide information about viral evolution. And in part 3, Hatfull describes work from his lab on mycobacteriophages, phage that infect mycobacteria including M. tuberculosis, the causative agent of the human disease tuberculosis. By studying mycobacteriophage, Hatfull hopes to better understand tuberculosis and perhaps develop methods for its control.
Graham Hatfull is Professor of Biotechnology and Chair of the Department of Biological Sciences at the University of Pittsburgh and a Howard Hughes Medical Institute Professor. He received his PhD from Edinburgh University and was a post-doctoral fellow with Nigel Grindley at Yale University and Fred Sanger at the MRC. His lab focuses on studying the bacteriophage that infect mycobacteria as a strategy to better understand the pathogen M. tuberculosis.
Hatfull is also the founder of the Phage Hunters Integrating Research and Education (PHIRE) program. In this program, college and high school students actively participate in identifying and characterizing bacteriophage allowing them to experience biological research first hand. Hatfull describes this program his talk in iBioMagazine.
- David Botstein iBioSeminar: Fruits of the genome sequences
- Graham Hatfull: Encouraging Scientific Curiosity
- Ari Helenius iBioSeminar: The cell biology of virus entry
- Stephen Harrison iBioSeminar: Viral structures
Hendrix RW, Smith MC, Burns RN, Ford ME, Hatfull GF. (1999). Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage. Proc Natl Acad Sci U S A. Mar 2;96(5):2192-7.
Hatfull GF, Pedulla ML, Jacobs-Sera D, Cichon PM, Foley A, Ford ME, Gonda RM, Houtz JM, Hryckowian AJ, Kelchner VA, Namburi S, Pajcini KV, Popovich MG, Schleicher DT, Simanek BZ, Smith AL, Zdanowicz GM, Kumar V, Peebles CL, Jacobs WR Jr, Lawrence JG, Hendrix RW. (2006). Exploring the mycobacteriophage metaproteome: phage genomics as an educational platform. PLoS Genet. Jun;2(6):e92
Hatfull GF. (2010) Mycobacteriophages: genes and genomes. Annu Rev Microbiol. Oct 13;64:331-56.
Hatfull GF, Jacobs-Sera D, Lawrence JG, Pope WH, Russell DA, Ko CC, Weber RJ, Patel MC, Germane KL, Edgar RH, Hoyte NN, Bowman CA, Tantoco AT, Paladin EC, Myers MS, Smith AL, Grace MS, Pham TT, O'Brien MB, Vogelsberger AM, Hryckowian AJ, Wynalek JL, Donis-Keller H, Bogel MW, Peebles CL, Cresawn SG, Hendrix RW. (2010) Comparative genomic analysis of 60 Mycobacteriophage genomes: genome clustering, gene acquisition, and gene size. J Mol Biol. Mar 19;397(1):119-43.
Hatfull GF. (2010) Bacteriophage Research: Gateway to Learning Science. Microbe 5 (6) 243-250.