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Confocal microscopy is a powerful technique for acquiring three-dimensional images of biological samples. Here I discuss the basic principles of confocal microscopy, with specific discussions of the operation of laser scanning and spinning disk confocal microscopes and of their application to biology.
Kurt Thorn received his Bachelor’s degree in chemistry from Princeton University, and his Ph.D. in Biophysics from UCSF. His graduate work was done in the laboratory of Dr. Ron Vale, studying kinesin processivity with single molecule assays. He then worked at Harvard as a Fellow in the Center for Genomics Research. Dr. Thorn is currently an Adjunct Assistant Professor at UCSF and director of the Nikon Imaging Center.
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