- There are struggles on a daily basis in science. Whether they be of a small magnitude, such as, your PCR didn't work or of a much larger magnitude, when you realize the project that you've been working on for the past six months is clearly coming to an end. - It's either that experiment that looked really great when you drew it out on the napkin doesn't actually work like at all or works so poorly that you don't think the data is reliable or you are trying to do something that someone else knows how to do but you haven't been able to replicate it or you're doing your experiments completely perfectly but the data doesn't make any sense, right? This happens to. No one gets to avoid these things. They happen to everybody in some proportion and if you here a story where those things are not featured it is because they are not being included in service of the story. It's not that they didn't happen. They happen every time. - I think it's important to remember that hardship in grad school can be both technical and conceptual and that there are ways around or ways through both as long as you are open to asking for help from many people but especially from your advisor. - We talk a lot or students are told a lot that they should have ownership of a project but that doesn't mean that you can't take advice from somebody else. There are students who feel the can't take advice from an advisor or from anyone else because then they will be relinquishing that ownership of the projects that's not usually something that's going to be helpful to them. - I've had students who feel like they can't ask for anything and they shouldn't get help from anyone because that's part of graduate school and really, trust me, it's not part of graduate school. It doesn't need to be part of graduate school. The scientific discipline as a whole realizes that you can't do everything so we need to come toegtehr to solve these problems. - There are different scales on which you can ask for help, right? When you learn to do something new in the lab, at least the way I always was taught to do things, was first I do it side by side with someone while I like take down notes then I do it by myself and if it doesn't work then I go back to the person to sort of reassess but that's already asking for help like at the very earliest stages of figuring out how to do something. There's nothing wrong with asking for help, it's called learning, right? Then there's sort of larger scale stuff like sometime cloning can look like this. You've got a batch of constructs that you want to make and there are some that like won't give way and it's like super annoying. There I think it's appropriate to sort of do things independently through the whole cycle a couple of times and then there's something wrong with the design. If it's not working you should be questioning why something routine isn't working. - My most difficult time in grad school wasn't due to technical hardship, it was due to conceptual hardship and what got me through it was just being open to verbalizing the problem to as many people as I could possibly find and taking in creative input in terms of people who could broaden out my idea of what would be a satisfying conclusion to that problem. So, Angela and I worked on this for a long timea dn it was basically her input that allowed me to see a different type of approach and actually an approach that I had taken much, much earlier on in a slightly different way was the answer to figuring out the way over that hurdle. - So realizing that dependence can beget independence. That the route to independence is ususally through dependence. - I think you need to be your biggest advocate. So, when I say that it means that ultimately you're in control of your future. If something's not right, if something doesn't feel like it should be going in a certain direction, it's up to you to come to the P.I. or come to your colleagues or your other mentors within the lab and really bring that up. I think so many times I've seen students, again regardless of the reason, think they just have to continue on a path until it works or until they graduate, not really realizing that they can take charge of their pathway within this project or within their graduate time. - The responsibility you have as a trainee is to be as diligent and as self-aware in how you are executing stuff in the lab such that when it doesn't work you can talk about it in a meaningful way, right? It's incredibly frustrating to have somebody dump a pile of disorganized stuff in front of you and tell you that it doesn't work because you don't know to help but if somebody's like, I did this protocol this way and the positive control looks okay but the negative control doesn't or whatever it is, that is something specific that you can help with. - There's this really pervasive and really damaging idea in science which is the myth of the lone genius. That everybody should be able to to carry out their projects or their research on their own and that you have these brilliant people somewhere in isolation coming up with these great ideas and answers to problems and it's just never true. - Being alone, certainly you can show that you can do something but it's a whole lot more fun to be a team member and also, I think, it allows you to be more productive and to really not feel like you, yourself have to learn everything. To learn from those around you. - I learned from every one and you can learn from every one. You learn from people who are slightly ahead of you in their career stage and you learn from people who have come in right behind you but I think one of the greatest things that you can do is listen to advice. Take in all the advice that you can and gather it from everyone. Some advice is going to fit you and other advice is not at all but if you can get guidance and mentorship from kind of a committee of people that you can surround yourself with. You may be able to figure out what is the best path for you.