By Anthony Hyman (Max Planck Institute of Cell Biology and Genetics, Dresden)
In his talk, Dr. Hyman discusses two methods: RNA interference and Mass Spectrometry (following immunoprecipitation).
Compare the type of information that each of these methods can produce when it comes to analyzing:
- A given protein’s function
- A given protein’s contribution to a particular protein complex
Organization of Cytoplasm
|Length: 27:58 minDownload: This Video|
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A eukaryotic cell is often 5-6 orders of magnitude larger than the molecules that make it up. How is it that these molecules interact to organize the complex structures that constitute a cell? In part 1 of his seminar, Dr. Hyman explains how cell division in a C. elegans embryo provides an excellent model for organization of cellular structures and processes and how RNA interference (RNAi) is an extremely useful tool to study this model. He describes how individual proteins can form complexes of varying size and complexity. Complexes can then organize to form compartments, or non-membrane bound organelles such as centrosomes or the cell cortex, and the organization of these compartments drives the organization of cells.
Anthony Hyman received his BSc in Zoology from University College, London. He did his first experiments on C. elegans as a PhD student in the Laboratory of Molecular Biology in Cambridge. He then moved to the University of California, San Francisco to pursue postdoctoral research with Tim Mitchison. In 1993, Hyman moved to the EMBL in Heidelberg and in 1999 he became a Director of the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden. Hyman’s lab studies the spatial control of the microtubule cytoskeleton and how this affects mitosis and cell division. Much of his research uses the technique of RNA interference in C. elegans embryos but some experiments study human cells using bacterial artificial chromosomes (BAC) transgenesis.
- Anthony Hyman iBioSeminar Part 2: Building a polymer: microtubule dynamics
- Anthony Hyman iBioSeminar Part 3: Formation and duplication of centrioles
- Anthony Hyman iBioSeminar Part 4: Formation of P granules
- Timothy Mitchison iBioSeminar Part 1: Self Organization in Biology