Resolution in Microscopy
|Length: 39:00 minDownload: High ResLow Res|
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|Description: This lecture describes the diffraction of light, a key principle in image formation and a factor that limits the resolution of a conventional light microscope. The behavior of light traveling through an objective is described along with the concept of numerical aperture. The “point spread function” (or PSF) and Nyquist sampling are explained, which are critical concepts for understanding image resolution and detection of images.|
|About the Speaker: Jeff Lichtman|
One of Dr. Lichtman’s research goals is to generate a complete map of all the neural connections in the brain- the “connectome”. His lab has developed several novel imaging techniques including the “Brainbow” mouse and serial electron microscopy reconstruction to reach this goal. Lichtman is the Jeremy R. Knowles Professor of Molecular and Cellular Biology and Santiago Ramón y Cajal Professor of Arts and Sciences at Harvard University.