Voeltz explains how her lab used a BioID strategy to identify some of the proteins found at membrane contact sites between the ER and endosomes; a difficult task given the transient nature of contact sites. They were able to identify a number of proteins responsible for marking the timing and location of endosome fission and for recruiting the ER to the bud. Depleting these proteins blocked cargo sorting to the Golgi. Voeltz’ lab is now working to determine how much of this machinery is conserved in processes such as the mitochondrial
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Endoplasmic Reticulum Membrane Contact Sites
The endoplasmic reticulum (ER) is a dynamic network of tubules that reaches throughout a cell. It interacts with other organelles at membrane contact sites. As Dr. Gia Voeltz explains, these sites are critical for Ca2+ regulation, lipid transport and defining sites of division for endosomes and other organelles. (Talk recorded in January 2019)
- Part 1: Factors and Functions of Organelle Membrane Contact SitesAudience:
- Student
- Researcher
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 00:24:33 - Part 2: Using BioID to Identify Membrane Contact Site FactorsAudience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 00:23:33