The SOS Response in Bacteria: Evelyn Witkin Witkin provides a historical perspective on how working at Cold Spring Harbor shaped her scientific career and led to the discovery of the SOS DNA damage response in bacteria.
Developing GFP as a Biological Marker: Martin Chalfie Chalfie describes the events, both serendipitous and insightful, that led to the discovery that the green fluorescent protein (GFP) could be used to track the expression and localization of proteins, thus revolutionizing modern cell biology.
Discovering Ribozymes: Tom Cech Cech describes the excitement of doing the experiments that led to the discovery that RNA can catalyze the cutting and rejoining of chemical bonds.
Discovering Phosphorylation: Edmond Fischer Edmond Fischer tells an engaging story of how he and Edwin Krebs were the first to observe reversible protein phosphorylation, a mechanism so ubiquitous, it influences almost every cellular process.
How Muscle Contracts: Hugh Huxley Huxley obtained the first definitive images showing that muscle was comprised of an interdigitating array of myosin and actin filaments that slid relative to each other during contraction.
The Dynamic Mitotic Spindle: Shinya Inoue Dr. Inoue recalls how, in 1947, he built a polarizing light microscope that allowed him to visualize, for the first time, the dynamic mitotic spindle in live cells.
Visualizing Synaptic Signaling: Thomas Reese Thomas Reese describes a decade of experiments with John Heuser which led to their theory of synaptic vesicle recycling and allowed them to directly visualize synaptic vesicles fusing with the plasma membrane.
SNURPs and Serendipity: Joan Steitz Steitz goes on to tell us of the importance of serendipity in her lab’s discovery of SNURPS, the small nuclear ribonucleoproteins that are key to the splicing of pre-messenger RNA.
Discovering Kinesin: Ron Vale Vale explains how doing science often follows a winding path with unexpected, sometimes wonderful surprises, and uses his own story to illustrate his point.
Unfolding the UPR: Peter Walter Proteins that are secreted from the cell or inserted into the plasma membrane, transit through the endoplasmic reticulum where they are properly folded and assembled and may undergo post-translational modification.