Bringing the World's Best Biology to You
Recent advances in optics and nanoparticle technology will soon allow us to observe single molecules inside living tissue. (Talk recorded in June 2015)
Ron Vale provides an overview of the historical contributions from light microscopy to the life sciences and an overview of current exciting developments. This talk is in the Short Microscopy Series. (Talk recorded in March 2012)
Ron Vale provides an overview of the historical contributions from light microscopy to the life sciences and an overview of current exciting developments. (Talk recorded in )
Nico Stuurman describes different light sources for microscopy and shows a few examples of light sources that are currently used in microscopes. (Talk recorded in April 2012)
Dr. Sven Truckenbrodt describes the development of X10 expansion microscopy for visualizing the smallest parts of cells, such as synaptic vesicles. (Talk recorded in June 2019)
This popular, free online microscopy course begins with basics of optics, proceeds through transmitted light microscopy, and covers many microscopy methods.
69 Talks
Ron Vale gives an overview of the iBiology Microscopy Course, a series of lectures, labs, and tips given by leaders in light microscopy. (Talk recorded in July 2013)
In this introductory lecture on light microscopy, Dr. Nico Stuurman describes the principles and properties of fluorescence microscopy. (Talk recorded in April 2012)
Ted Salmon discusses the principles of dark field, phase contrast, polarization, and (DIC) microscopy and examples of how these techniques are used for imaging. (Talk recorded in July 2012)
Jeff Lichtman describes resolution in microscopy and the diffraction of light, a key principle in image formation and a factor that limits the resolution of a conventional light microscope. (Talk recorded in December 2012)
Roger Tsien discusses correlating fluorescence with electron microscopy using miniSOG, a protein that can be imaged fluorescently and produces an electron-dense deposit. (Talk recorded in May 2012)
David Agard describes several methods for approximately doubling the resolution of the light microscope, including the technique of structured Illumination microscopy. (Talk recorded in July 2012)
Daniel Axelrod, pioneer of total internal reflection fluorescence microscopy (TIRF), describes this technique and explains the principles of the evanescent wave. (Talk recorded in May 2012)
Stefan Hell describes two super-resolution microscopy techniques: STED (Stimulated Emission Depletion) and RESOLFT (REversible Saturable OpticaL Fluorescence Transitions). (Talk recorded in May 2013)
In this talk about light sheet sectioning, Ernst Stelzer discusses the new technique of light sheet microscopy, also known as selective plane illumination (SPIM). (Talk recorded in May 2012)
In this introductory lecture on light microscopy, Dr. Nico Stuurman describes the principles and properties of fluorescence microscopy. (Talk recorded in April 2012)
Steve Ross illustrates the components in the optical light path and how they need to be aligned for phase microscopy, polarization microscopy, and DIC microscopy. (Talk recorded in January 2013)
Edward Salmon discusses polarization microscopy, which probes the interaction of molecules with polarized light, and describes the components of a polarization microscope. (Talk recorded in July 2012)
Shinya Inoue, one of the great innovators of polarization microscopy, describes polarized light and how light passing through a calcite crystal is split into two polarized light beams. (Talk recorded in July 2011)
iBiology and iBiology Courses are part of the Science Communication Lab (SCL). Our mission remains the same, to connect people to science. However, our focus has shifted to producing and evaluating cinematic films for education and the public, which you can find on the Science Communication Lab website. For more information, please see this blog post!
