Description: What if every person in the world could carry a microscope around in their pocket? That is the idea behind the foldscope, a 50-cent print-and-fold paper mass produced microscope. Foldscopes can be made to perform brightfield, darkfield, fluorescence, and polarization microscopy, and can reach submicron resolution. With applications ranging from global health to citizen science and K-12 education, Manu Prakash and his team’s goal is to get a foldscope in…
Length: 15:20 min Type: iBioMagazine Topic: Cell biology
Description: Selective Plane Illumination Microscopy (SPIM) greatly reduces phototoxicity in comparison with other fluorescent imaging modalities and makes it possible to image living small animals in 3D over extended periods of time. This talk describes an extension of SPIM such that it can be used with specimens on a coverslip rather than in a capillary (inverted SPIM or iSPIM), and a second modification that images the specimen using two perpendicular light…
Length: 20:11 min Type: iBioEducation Topic: Techniques
Description: During their 20-year friendship, Betzig and Hess worked together and separately, in academia and industry, before eventually joining forces to develop the first super-high-resolution PALM microscope. They tell us the story of this journey and emphasize how their unusual and varied backgrounds provided the skills to complete the project.
Length: 14:46 min Type: iBioEducation Topic: Biophysics
Description: This concluding lecture summarizes the main techniques covered in the short course and provides an overview of when a specific microscopy method might be advantageous for a particular sample or biological experiment.
Length: 29:18 min Type: iBioEducation Topic: Techniques
Description: This lecture describes how digital cameras for microscopes work, what a “pixel” is, Nyquist sampling, the dynamic range, noise, and color cameras. The features of an image is described, included bit-depth, intensity scaling and histograms, file formats and basic image processing.
Length: 33:41 min Type: iBioEducation Topic: Techniques
Description: Super-Resolution Microscopy: Xiaowei Zhuang: This lecture surveys a variety of recent methods that achieve higher resolution than is possible with conventional microscopy with diffraction-limited optics. These include different types of patterned illumination (e.g. STED and SIM microscopy) or techniques that build up an image by stochastically switching on single fluorescent molecules and localizing each molecule with high spatial precision (STORM, PALM, FPALM).
Length: 37:36 min Type: iBioEducation Topic: Techniques
Description: This lecture describes how fluorescence recovery after photobleaching (FRAP), fluorescence loss in photobleaching (FLIP) and photoactivation of fluorophores can provide information on the dynamics of molecules in cells. Uses of these techniques include studying the movement of proteins through membrane compartments, the diffusional behavior of molecules in the cytosol and membranes, the dynamics of cytoskeletal polymers, and protein turnover.
Length: 26:29 min Type: iBioEducation Topic: Techniques
Description: This talk introduces confocal microscopy, and discusses optical sectioning, reconstruction of 3D images, and how the laser-scanning confocal microscope and spinning disk confocal microscope work.
Length: 24:47 min Type: iBioEducation Topic: Techniques
Description: This lecture describes the chemical properties of organic dyes and quantum dots (Q dots) that make them fluoresce and how their fluorescence wavelength is tuned. It also describes how dyes can be attached to proteins, photobleaching, and other dyes that bind DNA (Hoeschst) or organelles (mitotracker, lysotracker).
Length: 20:48 min Type: iBioEducation Topic: Techniques
Description: This lecture describes various methods of generating contrast in biological specimens using transmitted light (i.e. without staining or fluorescence). Principles of dark field, phase contrast, polarization and differential interference contrast (DIC) microscopy are described and examples of how these techniques are used for imaging are described.
Length: 31:28 min Type: iBioEducation Topic: Techniques