This lecture provides an overview of the historical contributions that light microscopy has made to the life sciences and an overview of current exciting developments, such as GFP, super-resolution microscopy, using microscopy to measure biochemical reactions, and imaging of the nervous system.
- Which scientist coined the term “cells” from microscopic observations of slices of cork?
- Walther Flemming
- Robert Hooke
- Isaac Newton
- Antonie van Leeuvenhoek
- What statement is false about the Green Fluorescence Protein?
- Was first discovered and purified from a jelly fish
- Is not compatible with live cell imaging but is a substantial improvement over immunofluorescence
- Can be fused to proteins, allowing their fluorescence to be genetically encoded
- Will fluoresce in cells without the need for introducing other cofactors
- Early microscopists such as Camillo Golgi and Walther Flemming succeeded in visualizing new intracellular structures by:
- Utilizing stains for highlighting cellular structures.
- Improving the quality of objective lenses
- Pioneering the development of live cell imaging
- Developing sectioning techniques of cells
- Super-resolution light microscopy generally refers to:
- Improving the temporal resolution to the sub-millisecond regime
- Obtaining atomic resolution information on proteins
- Breaking the diffraction limit of spatial resolution (Abbe equation)
- All of the above