In this talk about light sheet sectioning, Ernst Stelzer discusses the new technique of light sheet microscopy, also known as selective plane illumination (SPIM). This uses two objectives, one to illuminate the sample and a second to image it, and allows long-term 3D imaging of thick specimens, like developing embryos, with minimal photobleaching and phototoxicity.
- What is the fundamental idea behind light sheet imaging?
- Why is phototoxicity and photobleaching reduced?
- What are the advantages of doing multiple rotation angles?
Dr. Stelzer trained as a physicist and has used his expertise to develop numerous optical techniques for studying problems in the life sciences. Currently his lab focuses on using light sheet-based fluorescence microscopy to study cells cultured in 3D. Stelzer is Vice-Director of the Buchmann Institute for Molecular Life Sciences and Professor in the Life… Continue Reading