To image living cells with a microscope, they must stay alive. Here we discuss how to control the temperature, humidity, and atmosphere (CO2 concentration) around your cells so that they survive during live cell imaging. We discuss both simple and more complex systems for environmental control.
Kurt Thorn is an Assistant Professor of Biochemistry and Biophysics at UCSF and Director of the Nikon Imaging Center – a facility that provides cutting edge light microscopy equipment to UCSF researchers. Kurt can be followed on his blog at http://nic.ucsf.edu/blog/. Continue Reading