Talk Overview
Single cell sequencing, as the name implies, allows researchers to examine the genomic information for individual cells. This provides an opportunity to study cell-to-cell differences and identify cell subtypes, which offers insight into how specific cells function within and respond to their environment. Dr. Eric Chow begins his talk with an overview of single cell sequencing with a focus on RNA. He then goes on to outline the predominant approaches, including plate-based, microfluidic-based, and combinatorial indexing methods. He finishes by addressing approaches to single cell analysis that don’t rely on RNA, including methods that use DNA, proteins, and antibodies. He also reviews some of the benefits and limitations of analysis at the level of individual cells.
Speaker Bio
Eric Chow
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS… Continue Reading
Nelly Mostajo says
Really clear and instructive talk, thank you!
I have some curiosities, are ncRNAs, specially sRNAs ,also possible to be sequenced using these methods? Can it be then used for the characterization of exososomes? Taking in account that one could combine the methods and then produce a read out of proteins/transcripts inside these vesicles?
Thank you!
Vinusha Wickramasinghe says
Interesting talk!