Drubin describes how his lab began studying actin dynamics and endocytosis in yeast using two-color, real-time fluorescence and kymographs. These tools enabled his team to confirm that actin regulated clathrin-mediated endocytosis. They discovered that BAR and F-BAR proteins stabilized the process by acting as a scaffold for actin and facilitating invagination and fission of the cell membrane to develop vesicles.
View the full talk with additional resources on our website
Actin Dynamics and Endocytosis in Yeast and Mammalian Cells
In this series of videos, Dr. David Drubin describes the critical link between actin dynamics and endocytosis in both budding yeast and mammalian cells. (Talk recorded in October 2017)
- Part 1: Introduction: Actin, Endocytosis and the Early Days of Yeast Cell BiologyAudience:
- Student
- Researcher
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 25:09 - Part 2: Actin Dynamics and Endocytosis in YeastAudience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 30:25 - Part 3: Actin Dynamics and Endocytosis in Mammalian CellsAudience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 35:12 - Part 4: Actin Assembly in Budding YeastAudience:
- Researcher
- Educators
- Educators of Adv. Undergrad / Grad
Duration: 28:37