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Home » Courses » Short Microscopy Series

Short Microscopy Series

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Series Directors: Ron Vale, Nico Stuurman, and Kurt Thorn

This 14 lecture series provides a good overview for the techniques of light microscopy. Leading scientists in light microscopy cover the basics of optics, transmitted light, fluorescence and GFP, digital imaging, and recent advances in super-resolution microscopy. The series is appropriate for undergraduates or anyone who is relatively new to microscopy.

Introduction

  • Light Microscopy Ron Vale

    Historical Contributions from Light Microscopy

  • History of Early Microscopes: Joe Gall.

    History of Early Microscopes

Transmitted Light Microscopy

  • Resolution in Microscopy (Jeff Lichtman)

    Resolution in Microscopy

  • Dark Field, Phase Contrast, Polarization and DIC Edward Salmon

    Darkfield, Phase Contrast, Polarization, and DIC Microscopy

  • Daniel Fletcher: Lenses and Image Formation

    Refractive Lenses and Image Formation

  • Microscopy: Microscope Imaging and Koehler Illumination (Ron Vale

    Microscope Imaging and Koehler Illumination

Collecting and Analyzing Images

  • Cameras and Digital Image Analysis: Nico Stuurman

    Cameras and Digital Image Analysis

Fluorescence Microscopy

  • Introduction to Fluorescence Microscopy

  • Timothy Mitchison Fluorescent Probes.jpg

    Fluorescent Probes: Organic Dyes and Quantum Dots

  • Microscopy: Optical Sectioning and Confocal Microscopy (Kurt Thorn)

    Optical Sectioning and Confocal Microscopy

  • Jennifer Lippincott-Schwartz

    Measuring Dynamics: Photobleaching and Photoactivation

  • Xiaowei Zhuang Part 1

    Super Resolution Microscopy

  • Microscopy: Fluorescent Proteins (Roger Tsien)

    Fluorescent Proteins and the Story Behind GFP

Collecting and Analyzing Images

  • Choosing the Right Microscopy Technique Ron Vale

    Choosing the Right Microscopy Technique

 

List of Speakers

Daniel Fletcher (UC Berkeley)
Joseph Gall (Carnegie Institution)
Jennifer Lippincott-Schwartz (NIH)
Jeff Lichtman (Harvard)
Timothy Mitchison (Harvard)
Edward Salmon (University of North Carolina)
Nico Stuurman (UCSF)
Roger Tsien (UCDS/HHMI)
Kurt Thorn (UCSF)
Ron Vale (HHMI/UCSF)
Xiaowei Zhuang (HHMI/Harvard)

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Funders

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NIGMS
Lasker
Rita Allen

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This material is based upon work supported by the National Science Foundation and the National Institute of General Medical Sciences under Grant No. 2122350 and 1 R25 GM139147. Any opinion, finding, conclusion, or recommendation expressed in these videos are solely those of the speakers and do not necessarily represent the views of the Science Communication Lab/iBiology, the National Science Foundation, the National Institutes of Health, or other Science Communication Lab funders.

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