This lecture covers the lenses of the microscope and how they are used to focus light onto a specimen and how light from the specimen is focused onto a camera or the retina of an eye. Koehler illumination, which provides even illumination of a sample, is described.
- List three planes in the microscope that are conjugate to image forming planes.
- List three planes in the microscope that are conjugate to the illumination (light source) plane
- Why do you want to set up Koehler illumination?
Ron Vale is a professor at the University of California, San Francisco and an Investigator of the Howard Hughes Medical Institute. His lab combines cell biological and biophysical approaches to understand spatial organization, movement, and signaling within cells. Vale is a member of the National Academy of Sciences and a winner of the 2012 Lasker… Continue Reading