Dr. Malte Paulsen gives an introduction to flow cytometry with an excellent explanation of the basic principles governing the technique. He explains how fluid flow is used to focus a sample in a laser beam. Light from the laser is scattered by cells in the sample and the degree of scatter provides information about the cell’s optical density and other characteristics. In conventional flow cytometry, lasers are used primarily to excite fluorescent antibodies bound to specific cell types. A detector with different filters allows specific wavelengths to be dissected from the overall fluorescence. This signal can then be displayed in ways that provide the most information about the cell type of interest.
Dr. Malte Paulsen has been Head of the Flow Cytometry Core Facility at EMBL since 2015. Prior to joining EMBL, Paulsen was Head of the flow cytometry facilities first at Institute for Molecular Biology (IMB) in Mainz, and later at the National Heart and Lung Institute, Imperial College, London. Paulsen received his PhD from the… Continue Reading
2 raised to four equals 16 channels, not 8 as shown in the Flow cytometry talk. Thanks.
Mykhailo Tolkachov says
Splendid work at explaining this extremely relevant method!
I wish I could see more videos from Mr Paulsen in the future. Greetings from the LMU, Munich!
By the way, though I am not sure whether it is an appropriate place to leave a question,
what would be a good way to quantify the apoptotic cells by FACS and what dye would be used in this case?
Either way, thank you for your work.