Course Directors: Ron Vale, Nico Stuurman, and Kurt Thorn
Light microscopy has become one of the most useful tools in the life sciences. This free online comprehensive series begins with the basics of optics, proceeds through transmitted light microscopy, covers the various methods of imaging fluorescent samples, describes how cameras work and image processing, and concludes with some of the latest advances in light microscopy. In addition to lectures, we also provide labs (filmed at a microscope) and short tips, so as to cover pragmatics of how to use microscopes. Assessments are provided for each lecture. Enjoy learning microscopy! Note that each section header is a link and will lead to additional videos not displayed on this page.
SEE FULL TABLE OF CONTENTS FOR THE SERIES >>
Introduction
Image Formation
Refractive Lenses and Image Formation
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Objective Lenses and Eyepieces
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Huygens Wavelets Constructive/Destructive Interference, and Diffraction
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Point Spread Function
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Resolution of a Microscope
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Microscope Imaging and Koehler Illumination
Contrast Generation for Transmitted Light
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Darkfield and Phase Contrast Microscopy
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Polarized Light and its Interaction with Material
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Polarization Microscopy
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Differential Interference Contrast (DIC) Microscopy
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Examples of Using Polarization Microscopy
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Phase, Polarization, and DIC Microscopy Lab
Fluorescence Microscopy
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Introduction to Fluorescence Microscopy
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Fluorescent Probes: Organic Dyes and Quantum Dots
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Fluorescent Proteins and the Story Behind GFP
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Fluorescent Protein Indicators
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Two-Photon Microscopy
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Light Sheet Sectioning
Robotics, Detection and Image Analysis
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Cameras and Detectors I: How Do They Work?
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Cameras and Detectors II: Specifications and Performance
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Introduction to Digital Images
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Image Analysis
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Deconvolution Microscopy
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Using Software to Control Microscopes
Specialized Microscopy
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Optical Traps: An Introduction
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High Throughput Microscopy
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Array Tomography
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Miniature Microscopes for Deep Tissue Imaging
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Optogenetics: The Use of Opsin Genes in Technique Development
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Mobile Microscopy
List of Speakers
Daniel Axelrod (University of Michigan)
Philippe Bastiaens (Max Planck Institute)
Carlos Bustamante (UC Berkeley/HHMI)
Karl Deisseroth (Stanford/HHMI)
Jan Ellenberg (European MBL)
Daniel Fletcher (UC Berkeley)
Joseph Gall (Carnegie Institution)
Stefan Hell (Max Planck Institute)
Bo Huang (UCSF)
Shinya Inoue (MBL)
Jennifer Lippincott-Schwartz (NIH)
Jeff Lichtman (Harvard)
Timothy Mitchison (Harvard)
Stephen Ross (Stanford)
Edward Salmon (University of North Carolina)
Mark Schnitzer (Stanford/HHMI)
Stephen Smith (Stanford)
Nico Stuurman (UCSF)
Roger Tsien (UCDS/HHMI)
Kurt Thorn (UCSF)
Ron Vale (HHMI/UCSF)
Clare Waterman (NIH)
Xiaowei Zhuang (HHMI/Harvard)
