Lectures
Introduction to Fluorescence Microscopy
Fluorescent Probes: Organic Dyes and Quantum Dots
Fluorescent Proteins and the Story Behind GFP
Fluorescent Protein Indicators
Optical Sectioning and Confocal Microscopy
Two-Photon Microscopy
Light Sheet Sectioning
Overview and Stimulated Emission Depletion (STED) Microscopy
Total Internal Reflection Fluorescence (TIRF) Microscopy
Super-Resolution: Localization Microscopy
Super-Resolution: Structured Illumination Microscopy (SIM)
Measuring Dynamics: Photobleaching and Photoactivation
Measuring Dynamics: Fluorescent Speckle Microscopy
Förster Resonance Energy Transfer (FRET) Microscopy
Fluorescence Lifetime Imaging Microscopy
Summary: Designing a Fluorescence Microscopy Experiment
Dual-View Inverted Selective Plane Illumination (diSPIM)