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Home » Courses » Microscopy Series » Fluorescence Microscopy

Fluorescence Microscopy

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Lectures

  • Introduction to Fluorescence Microscopy

  • Timothy Mitchison Fluorescent Probes.jpg

    Fluorescent Probes: Organic Dyes and Quantum Dots

  • Roger Tsien: Fluorescent Proteins

    Fluorescent Proteins and the Story Behind GFP

  • Roger Tsien Fluorescent Protein Indicators

    Fluorescent Protein Indicators

  • Microscopy: Optical Sectioning and Confocal Microscopy (Kurt Thorn)

    Optical Sectioning and Confocal Microscopy

  • Microscopy: Two Photon Microscopy (Kurt Thorn

    Two-Photon Microscopy

  • Light Sheet Sectioning (Ernst Stelzer)

    Light Sheet Sectioning

  • Stefan Hell

    Overview and Stimulated Emission Depletion (STED) Microscopy

  • Total Internal Reflection Fluorescence (TIRF) Microscopy (Daniel Axelrod)

    Total Internal Reflection Fluorescence (TIRF) Microscopy

  • Super-Resolution: Localization Microscopy (Bo Huang)

    Super-Resolution: Localization Microscopy

  • Super-Resolution: Structured Illumination Microscopy

    Super-Resolution: Structured Illumination Microscopy (SIM)

  • Jennifer Lippincott-Schwartz

    Measuring Dynamics: Photobleaching and Photoactivation

  • Microscopy: Quantitative Analysis of Speckle Microscopy: Clare Waterman

    Measuring Dynamics: Fluorescent Speckle Microscopy

  • Forster Resonance Energy Transfer (FRET) Microscopy (Philippe Bastiaens)

    Förster Resonance Energy Transfer (FRET) Microscopy

  • Philippe Bastiaens Fluorescence Lifetime Imaging Microscopy

    Fluorescence Lifetime Imaging Microscopy

  • Microscopy: Designing a Fluorescence Microscopy Experiment (Kurt Thorn

    Summary: Designing a Fluorescence Microscopy Experiment

  • Hari Shroff

    Dual-View Inverted Selective Plane Illumination (diSPIM)

Labs

  • Disassembling a Nikon Ti Eclipse Stephen Ross

    Demystifying Microscopes: Disassembling a Nikon Ti Eclipse Microscope

  • Nico Sturman on iBiology: Disassembling and ASI Ramm in Microscopy

    Demystifying Microscopes: Disassembling an ASI RAMM Microscope

Tips

  • Optimizing Detection of GFP

  • Ron Vale

    Minimizing Damage from Fluorescence

  • Microscopy: Quantitative Analysis of Speckle Microscopy: Clare Waterman

    Quantitative Analysis of Speckle Microscopy

  • Microscopy: Live Cell Imaging and Environmental Control (Kurt Thorn

    Live Cell Imaging and Environmental Control

Bonus Lecture

  • Labeling Proteins with Fluorescent Probes (Timothy Mitchison)

    Labeling Proteins with Fluorescent Probes

  • Correlating Fluorescence With Electron Microscopy Roger Tsien

    Correlating Fluorescence with Electron Microscopy

  • Steven Chu

    Pushing the Boundaries of Light Microscopy

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This material is based upon work supported by the National Science Foundation and the National Institute of General Medical Sciences under Grant No. 2122350 and 1 R25 GM139147. Any opinion, finding, conclusion, or recommendation expressed in these videos are solely those of the speakers and do not necessarily represent the views of the Science Communication Lab/iBiology, the National Science Foundation, the National Institutes of Health, or other Science Communication Lab funders.

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