Fluorescent speckle microscopy is a technique that allows monitoring of dynamics in polymeric structures by doping in a very low level of fluorescently labeled monomer. The small number of fluorescent molecules make fluorescent speckles that show up as diffraction-limited bright spots in the image. Here, Clare Waterman, the inventor of this technique, describes how to make and image speckled samples.
- The optimal ratio of labeled protein to unlabeled protein for speckle microscopy is about?
- 1 : 100 000
- 1 : 100
- 1 : 10
- 100 : 1
- Speckled microtubules
- Result from random incorporation of labeled protein
- Can only be seen in vivo
- Result from structural variation of the microtubule
- Result from binding of other proteins to the microtubule
- Which of the following CANNOT be measured with speckle microscopy:
- The rate of binding of labeled protein
- The trajectory and velocity of labeled protein
- The rate of dissociation of labeled protein
- Overall structure and distribution of labeled protein
- Which of the following objectives would you choose for speckle microscopy?
- Plan Apo 10x/0.45
- Plan Apo 40x/0.95
- Plan Apo 40x/1.15 water immersion
- Plan Apo 100x/1.4
Dr. Waterman is chief of the Laboratory of Cell and Tissue Morphodynamics at the National Heart Lung and Blood Institute, National Institutes of Health and she has been co-director of the Physiology Course at the Marine Biological Laboratory for the past 4 years. Waterman’s lab studies the interactions between actin and focal adhesions, taking advantage… Continue Reading