Ron Vale illustrates how illumination of a specimen can cause photobleaching and protein damage and gives us a tip about minimizing damage from fluorescence. Ways to minimize such problems include strategies for minimizing light exposure and removing molecular oxygen from in vitro reactions.
Ron Vale is a professor at the University of California, San Francisco and an Investigator of the Howard Hughes Medical Institute. His lab combines cell biological and biophysical approaches to understand spatial organization, movement, and signaling within cells. Vale is a member of the National Academy of Sciences and a winner of the 2012 Lasker… Continue Reading