In his second talk, Haber explains in greater detail the molecular steps that take place during the repair of a DNA double strand break. It turns out that the process of mating type switching in S. cerevisiae requires site-specific cutting and repair of a yeast chromosome and this is an excellent model for studying DNA DSB repair. Working in this system and using techniques such as Southern blots, PCR and chromatin immunoprecipitation, Haber’s group was able to identify the proteins and enzymatic steps in DNA repair.
View the full talk with additional resources on our website
Broken Chromosome Repair by Homologous Recombination
Broken chromosomes naturally arise during DNA replication. In healthy cells, the breaks are repaired by homologous recombination. If the repair machinery is broken, cancer can result. (Talk recorded in October 2018)
- Part 1: Broken Chromosome Repair by Homologous RecombinationAudience:
- Student
- Researcher
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 00:35:59 - Part 2: Molecular Mechanisms of Repairing a Broken ChromosomeAudience:
- Student
- Researcher
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 00:33:14 - Part 3: Mutations Arising during Repair of a Broken ChromosomeAudience:
- Student
- Researcher
- Educators
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 00:23:01