In his second talk, Peters presents evidence that cohesin is indeed necessary for genomic DNA to fold into loops. Long range DNA interactions such as loops can be detected using a technique called Hi-C. Using Hi-C, Peters shows that depleting cohesin removes DNA loops, while depleting the proteins that remove cohesin from DNA, results in bigger DNA loops. In addition, CTCF appears to recognize specific sequences that define the base of the loops. Incorporating all of this data, Peters describes a model in which DNA is extruded by cohesin to form a loop and the boundaries of the loop are determined by CTCF. Peters explains that many questions about the mechanism of DNA loop extrusion and its importance in cells remain to be answered.
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How do Cohesin and CTCF Fold DNA in Mammalian Genomes?
The cohesin protein complex joins sister chromatids together before they are segregated during mitosis. In this talk, Dr. Peters presents evidence that cohesin also plays an important role in DNA folding and genome organization. (Talk recorded in May 2019)
- Part 1: Cohesin: Roles Beyond Sister Chromatid Cohesion?Audience:
- Student
- Researcher
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 00:33:13 - Part 2: How do Cohesin and CTCF Fold DNA in Mammalian Genomes?Audience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 00:36:34