Proteins that are secreted from the cell or localized to the plasma membrane need first to be translocated into the lumen of the ER or inserted into the ER membrane. Thousands of proteins, each with a unique signal sequence, move through this pathway. How does the protein translocation machinery recognize these diverse signals and correctly localize the protein? In his third talk, Hegde describes studies from his lab using cryo-electron microscopy to visualize the translocation machinery at different stages in the recognition and engagement of a secreted or membrane inserted protein. The structural information gleaned from these experiments helps to explain how the protein translocation machinery works with high fidelity even when it needs to recognize diverse signal sequences.
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Protein Localization Inside Cells
How does the cell regulate protein localization to be sure that proteins end up where they should? Manu Hegde reviews experiments that answer this question. (Talk recorded in April 2017)
- Part 1: Compartmentalization of Proteins Inside CellsAudience:
- Student
- Researcher
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 00:43:08 - Part 2: Quality Control of Protein LocalizationAudience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 00:34:09 - Part 3: Recognition of Protein Localization SignalsAudience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 00:46:27