Cheng shows how his laboratory has used Cryo-EM to study the atomic resolution of membrane proteins. It is challenging to use conventional methods to study membrane protein structure, given that the 3D structure of most membrane proteins is dependent on their interaction with the phospholipid bilayer. Cheng describes how his laboratory has overcome these challenges to successfully solve the protein structure of the TRPV1 ion channel in different conformations at atomic resolution. He describes the benefits of using of amphipols, lipid nanodiscs, and Fab-assisted approaches to facilitate structural studies.
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Single Particle Cryo-EM
Yifan Cheng overviews the principles of Cryo-EM, and describes how advances in this technique have allowed scientists to solve biological structures to atomic resolution. (Talk recorded in May 2017)
- Part 1: Single Particle Cryo-EMAudience:
- Student
- Researcher
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad
Duration: 00:34:07 - Part 2: Single Particle Cyro-EM of Membrane ProteinsAudience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 00:36:14