In in situ hybridization, RNA or DNA molecules can be visualized in an immobilized cell, tissue, or tissue section. This technique, which was pioneered by Joseph Gall in 1968, works by hybridizing a labeled RNA or DNA molecule that is complementary to the RNA or DNA of interest. In his talk, Gall describes the experiments that inspired him to develop this technique, and the key experiments that led to the first visualization of RNA and DNA inside the cell.
Joseph Gall is a Professor at the Carnegie Institution for Science and is a leader in the field of cell biology. He has spent much of his career studying the nucleus and its contents, and has made numerous significant contributions to our understanding of chromosome structure and function. Gall, a distinguished microscopist, is one of the… Continue Reading