I. Protein Synthesis: a High Fidelity Molecular Event
II. mRNA Surveillance by the Ribosome
Part I: Protein Synthesis: a High Fidelity Molecular Event
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In her first talk, Green provides a detailed look at protein synthesis, or translation. Translation is the process by which nucleotides, the “language” of DNA and RNA, are translated into amino acids, the “language” of proteins. Green begins by describing the components needed for translation; mRNA, tRNA, ribosomes, and the initiation, elongation, and termination factors. She then explains the roles of these players in ensuring accuracy during the initiation, elongation, termination and recycling steps of the translation process. By comparing translation in bacteria and eukaryotes, Green explains that it is possible to determine which components and steps are highly conserved and predate the divergence of different kingdoms on the tree of life, and which are more recent adaptations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRNAs and trigger events leading to the degradation of the bad RNA and the incompletely translated protein product and to the recycling of the ribosome components. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the protein Dom34 is critical for facilitating ribosome release from the short mRNAs that result from mRNA cleavage. Experiments showed that Dom34-mediated rescue of ribosomes from short mRNAs is an essential process for cell survival in higher eukaryotes.
Rachel Green received her BS in chemistry from the University of Michigan. She then moved to Harvard to pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their implications for the evolution of life. As a post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRNA to protein with such accuracy.
Currently, Green is a Professor of Molecular Biology and Genetics at the Johns Hopkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her lab continues to focus on the ribosome and factors involved in the fidelity of eukaryotic and prokaryotic translation.
Green is the recipient of a Johns Hopkins University School of Medicine Graduate Teaching Award as well as the recipient for numerous awards for her research. She was elected to the National Academy of Sciences in 2012.
- Ramanujan Hegde iBioSeminar: Protein Localization Inside Cells
- Roy Parker iBioSeminar: The Life of Eukaryotic mRNA
- Anna Marie Pyle iBioSeminar: RNA structure, Function and Recognition
- Randy Schekman iBioSeminar: The Secretory Pathway
- Robert Tjian iBioSeminar: The Molecular Biology of Gene Regulation
- Jack Szostak iBioSeminar: The Origin of Life on Earth
Craig N, Green R, Grieder C, Storz G, Wolberger C, and Cohen-Fix O. Molecular Biology Principles of Genome Function, 2nd Edition, Oxford University Press (2014) Chapters 11 and 12.
Shoemaker, CJ, Eyler, DE, Green, R. (2010) Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. Science 330: 369-72.
Shoemaker, CJ, Green, R. (2011). Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast. Proc Natl Acad Sci USA 108(51):E1392-8.
Shoemaker, CJ and Green, R. (2012) Translation drives mRNA quality control. Nat Struct Mol Biol 19:594-601.
Guydosh, NR and Green, R. (2014) Dom34 rescues ribosomes in 3' untranslated regions. Cell 156:950-62.