Dr. Susanne Heck begins her talk by explaining why we might choose to use mass cytometry rather than other types of flow cytometry. Traditional flow cytometry is typically limited to the detection of about a dozen parameters in one sample due to overlap between the emission spectra of fluorochromes used to label antibodies. Mass cytometry, on the other hand, allows for the detection of up to 50 parameters in one sample because antibodies are labelled with metal isotopes and separated based on their mass. Heck goes on to explain which metal isotopes are typically used for mass cytometry and why, and she describes how a mass cytometer functions. She finishes by running through an example of using mass cytometry to perform functional phenotyping on human bone marrow cells.
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How does mass cytometry differ from other types of flow cytometry? When would you choose to use it? How does a mass cytometer work? Dr. Susanne Heck gives an overview of mass cytometry and answers all of these questions. (Talk recorded in February 2019)
- Educators of H. School / Intro Undergrad
- Educators of Adv. Undergrad / Grad