Part 2: Cyclin-dependent kinases (Cdks) are the central components of the control system that initiates the events of the cell cycle. In the second part of this lecture, I discuss my laboratory’s efforts to address the problem of how the Cdks trigger cell-cycle events. I describe our methods for identifying the protein substrates of the Cdks, and I discuss how these studies have led to important clues about how Cdks find their correct targets in the cell and how phosphorylation of those targets governs their function.
Part 3: In the anaphase stage of the cell cycle, the duplicated chromosomes are pulled apart by a machine called the mitotic spindle, resulting in the distribution of a complete set of chromosomes to each of the daughter cells. In the third part of this lecture, I describe the combination of biochemistry and microscopy in my laboratory that led to the discovery of a regulatory switch that triggers the abrupt and synchronous separation of the chromosomes at the onset of anaphase.
David Morgan is a Professor in the Departments of Physiology and Biochemistry & Biophysics at the University of California, San Francisco (UCSF). He received an undergraduate degree in animal physiology from the University of Calgary in 1980, followed in 1986 by a PhD from UCSF, with Richard Roth. Following postdoctoral studies with William Rutter and… Continue Reading