When executing complex microscopy experiments, it is important to tightly synchronize the operation of camera(s), shutters, stages, etc.. This is usually done in software, however, current computer software has limits in its ability to respond quickly. This video shows how hardware synchronization, using TTL signals running through BNC cables, can dramatically speed up microscope image acquisition and reduce light exposure to biological samples.
Nico Stuurman is a Research Specialist at the University of California, San Francisco, in the lab of Ron Vale. Nico combines his expertise in computer programming and microscopy to advance many projects including the Open Source software, Micro-Manager. Continue Reading