Neurons are connected to form complex networks by tiny junctions called synapses. Svoboda reviews the process of synaptic transmission and the methods traditionally used to image neurons and their synapses. He then describes powerful new methodologies that allow synaptic transmission and calcium signaling in individual synapses to be measured in intact tissue. This lecture is suitable for neuroscience graduate students.
In the second part of this lecture, Svoboda focuses on how the imaging techniques introduced in his first lecture can be used to study calcium signaling and downstream pathways such as Ras, at the level of single synapses. This lecture is also suitable for neuroscience graduate students.
Karel Svoboda is a group leader at The Howard Hughes Medical Institute’s Janelia Farm Research Campus. His lab is studying synaptic plasticity in response to new experiences in the developing and adult neocortex. By developing new biochemical tools and new microscopes, Svoboda’s group has been able to track changes in individual synapses in intact tissue…. Continue Reading