• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar
  • Skip to footer

iBiology

Bringing the World's Best Biology to You

  • Start Here
    • Explore iBiology
    • All Playlists
    • All Speakers
    • All Talks
    • What’s new at iBiology?
  • Research Talks
    • Talks by Topic
      • Biochemistry
      • Bioengineering
      • Biophysics
      • Cell Biology
      • Development and Stem Cells
      • Ecology
      • Evolution
      • Genetics and Gene Regulation
      • Human Disease
      • Immunology
      • Microbiology
      • Neuroscience
      • Plant Biology
      • Techniques
      • Archive
    • Talks by Series
      • Bench to Bedside
      • Famous Discoveries
      • Great Unanswered Questions
      • Microscopy Series
      • Share Your Research Series
  • Stories
    • Background to Breakthrough
    • Interviews and Profiles
    • Science and Society
  • Courses
  • Careers
    • Professional Development Talks
    • Professional Development Courses
    • Career Exploration
    • NRMN Resources
    • Biomedical Workforce
  • Educators
  • About
    • Mission
    • iBiology Team
    • Board of Directors
    • iBiology Funders and Partnerships
    • Contact Us
  • Donate
Home » Courses » Microscopy Series » Image Formation

Eyepieces

  • Duration: 2:56
  • Downloads
    • Hi-Res
    • Low-Res
  • Subtitles
    • English
  • Transcript

01:00:13.15 something that is often taken for granted
01:00:15.13 in the microscope, and that is the eyepieces
01:00:17.10 of the microscope.
01:00:18.08 Oftentimes, we're not even actually using eyepieces,
01:00:21.03 nowadays. Looking directly at the image
01:00:24.10 on the camera, or other imaging device,
01:00:26.28 rather than in the eyepiece.
01:00:29.09 However, eyepieces are fairly important
01:00:31.28 and when you're looking
01:00:32.27 for the specimen of interest,
01:00:35.09 you should know how to properly
01:00:36.15 adjust the eyepieces
01:00:37.15 and know what they do.
01:00:38.19 So the eyepieces don't actually
01:00:41.08 contribute to the resolution
01:00:42.29 of the optical system.
01:00:43.27 They magnify the image
01:00:45.22 and relay it to the retina in your eye.
01:00:48.12 And they do determine
01:00:50.27 the field of view of the microscope
01:00:52.22 to your eye.
01:00:54.04 They're also the place
01:00:56.23 where if you had a reticle
01:00:58.01 or a mask, something that you wanted to
01:01:00.08 put a scale, for example, in,
01:01:02.03 would be located in the eyepiece.
01:01:04.26 One point I want to make
01:01:06.12 about eyepieces is that most eyepieces
01:01:08.14 nowadays are termed what they call
01:01:10.10 "high eye point eyepieces."
01:01:12.16 And what that means is
01:01:13.24 they're designed for people
01:01:15.07 with or without eyeglasses.
01:01:17.05 So you don't have to take your eyeglasses off
01:01:20.09 when you're looking in the eyepiece
01:01:22.03 of the microscope.
01:01:23.01 They're designed for that.
01:01:25.08 Useful magnification of the microscope
01:01:28.08 and system based on the NA
01:01:30.06 is about 500 to 1000 times.
01:01:32.12 So there are conjugate planes
01:01:36.06 within the eyepiece itself.
01:01:37.22 But the important thing that you want
01:01:39.22 to get from an eyepiece
01:01:40.19 is that you have a field plane,
01:01:42.16 here in the middle,
01:01:43.07 and there's actually a shelf there
01:01:45.13 that normally you can access
01:01:46.29 with a little ring that would put
01:01:49.14 a reticle, or an eyepiece reticle.
01:01:51.11 So you'd unscrew that ring
01:01:52.14 drop in your reticle,
01:01:53.23 and screw it back down tight.
01:01:55.15 The eyepiece actually produces
01:01:58.06 a virtual image, which is 250mm
01:02:02.05 away from the eye.
01:02:04.24 Or appears 250mm away.
01:02:06.27 That is sort of defined as 1X
01:02:10.09 to the human eye.
01:02:12.09 And was based actually
01:02:13.16 on the comfortable reading distance
01:02:16.06 of the human eye.
01:02:16.28 And the light from there,
01:02:19.19 you can see, goes through the eyepiece
01:02:21.12 and actually uses the lens in the eye
01:02:25.01 to reflect the image focused back on the retina.
01:02:28.25 So that's just a short tip on the eyepieces.

This Talk
Speaker: Steve Ross
Audience:
  • Researcher
Recorded: March 2012
More Talks in Microscopy Series
  • Steve Ross objective lens
    Features of an Objective Lens
  • How To Clean an Objective Lens (Kurt Thorn)
    How to Clean Objective Lenses and Filters
  • Stephen Ross
    Cleaning a Microscope: How to Find Dirt in Your Optical System
All Talks in Microscopy Series
Share

Talk Overview

In this talk about eyepieces, Stephen Ross gives a short description of what is in an eyepiece and how it works.

Speaker Bio

Steve Ross

Steve Ross

Stephen Ross is the General Manager of Product and Marketing at Nikon Instruments.  He is also very involved in teaching microscopy at the Marine Biological Laboratory in Woods Hole and at the Bangalore Microscopy Course at the National Centre for Biological Sciences. Continue Reading

Playlist: Microscopy Series

  • Measuring The Point Spread Function Nico Stuurman
    Measuring a Point Spread Function
  • Steve Ross objective lens
    Features of an Objective Lens
  • How To Clean an Objective Lens (Kurt Thorn)
    How to Clean Objective Lenses and Filters
  • Stephen Ross
    Cleaning a Microscope: How to Find Dirt in Your Optical System

Reader Interactions

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Primary Sidebar

Like our Website?

You'll love our newsletter...

  • Sign up for:
    • Exclusive iBiology content
    • 10 Must See Biology Videos
    • Talks by 20+ Nobel Winners
    • Our young Scientist Survival Toolkit
    • Talks by trailblazing Women in Biology
    • Updates on exciting iBiology projects in development
    Tyler Allen
  • This field is for validation purposes and should be left unchanged.

Privacy Policy

Help us keep bringing the world’s best biology to you!

Footer

Funders

National Science Foundation: NSF
Lasker Foundation
NIGMS

Partners

EMBL
EMBO
HHMI
NRMN
UCSF

Start Here

  • Talks for Everyone
  • Talks for Students
  • Talks for Research
  • Talks for Educators

Explore

  • Explore
  • All Playlists
  • All Speakers
  • All Talks

Talks By Topic

  • Biochemistry
  • Bioengineering
  • Biophysics
  • Cell Biology
  • Development and Stem Cells
  • Ecology
  • Genetics and Gene Regulation
  • Human Disease
  • Immunology
  • Microbiology
  • Neuroscience
  • Plant Biology
  • Techniques

Talks by Series

  • Bench to Bedside
  • Famous Discoveries
  • Great Questions
  • Share Your Research Series

Career

  • Professional Development
  • Career Exploration
  • NRMN Resources
  • Biomedical Workforce

Courses

  • Microscopy Series
  • Short Microscopy Series
  • Open edX Courses
  • Cell Biology Flipped Course
  • Engineering Life Flipped Course
  • Evolution Flipped Course

Educator

  • Educator Registration
  • Educator Resources
  • Log In

About Us

  • About Us
  • iBiology Team
  • Wonder Collaborative
  • Contact Us
  • Mission
  • Privacy Policy
  • SCL Financial Conflict of Interest Policy

This material is based upon work supported by the National Science Foundation and the National Institute of General Medical Sciences under Grant No. 2122350 and 1 R25 GM139147. Any opinion, finding, conclusion, or recommendation expressed in these videos are solely those of the speakers and do not necessarily represent the views of the Science Communication Lab/iBiology, the National Science Foundation, the National Institutes of Health, or other Science Communication Lab funders.

© 2022 - 2006 iBiology · All content under CC BY-NC-ND 3.0 license · Privacy Policy · Terms of Use · Usage Policy
 

Power by iBiology