Clare Waterman, developer of fluorescent speckle microscopy, describes computational tools (developed by Gaudenz Danuser) for automatic quantitative analysis of speckle microscopy data. (Talk recorded in July 2012)
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Clare Waterman, developer of fluorescent speckle microscopy, describes computational tools (developed by Gaudenz Danuser) for automatic quantitative analysis of speckle microscopy data. (Talk recorded in July 2012)
Roger Tsien discusses correlating fluorescence with electron microscopy using miniSOG, a protein that can be imaged fluorescently and produces an electron-dense deposit. (Talk recorded in May 2012)
Kurt Thorn describes factors to consider when designing a fluorescence microscopy experiment including choice of microscope and choice of dye or fluorescent protein. (Talk recorded in April 2012)
Philippe Bastiaens discusses fluorescence lifetime imaging (FLIM), the microscopes used for this technique, and some biological applications of FLIM. (Talk recorded in May 2012)
Philippe Bastiaens describes the physics behind Förster Resonance Energy Transfer (FRET) microscopy and how FRET can be measured with a microscope. (Talk recorded in May 2012)
Clare Waterman, the inventor of fluorescent speckle microscopy, describes how to prepare samples for speckle microscopy and how to image them. (Talk recorded in July 2012)
David Agard describes several methods for approximately doubling the resolution of the light microscope, including the technique of structured Illumination microscopy. (Talk recorded in July 2012)
Bo Huang describes super-resolution localization microscopy techniques such as STORM, PALM, FPALM, and GSDIM, and explains how to extend these techniques to 3D imaging. (Talk recorded in April 2012)
Daniel Axelrod, pioneer of total internal reflection fluorescence microscopy (TIRF), describes this technique and explains the principles of the evanescent wave. (Talk recorded in May 2012)
Stefan Hell describes two super-resolution microscopy techniques: STED (Stimulated Emission Depletion) and RESOLFT (REversible Saturable OpticaL Fluorescence Transitions). (Talk recorded in May 2013)
Kurt Thorn introduces two-photon microscopy which uses intense pulsed lasers to image deep into biological samples, including thick tissue specimens or even inside of live animals. (Talk recorded in April 2012)
Kurt Thorn introduces confocal microscopy and discusses optical sectioning, reconstruction of 3D images, and how different confocal microscopes work. (Talk recorded in June 2009)
In this introductory lecture on light microscopy, Dr. Nico Stuurman describes the principles and properties of fluorescence microscopy. (Talk recorded in April 2012)
Steve Ross illustrates the components in the optical light path and how they need to be aligned for phase microscopy, polarization microscopy, and DIC microscopy. (Talk recorded in January 2013)
Ted Salmon gives a lecture about the pragmatics of differential interference contrast (DIC) microscopy and video-enhanced contrast microscopy. (Talk recorded in July 2012)
Shinya Inoue describes examples of using polarization microscopy to image living biological organisms, including watching processes of mitosis. (Talk recorded in July 2011)
Ted Salmon discusses the mechanism of the differential interference contrast (DIC) Wollaston prisms along with how to generate optimal contrast. (Talk recorded in July 2012)
Edward Salmon discusses polarization microscopy, which probes the interaction of molecules with polarized light, and describes the components of a polarization microscope. (Talk recorded in July 2012)
Ted Salmon describes the principles of dark field and phase contrast microscopy, two ways of generating contrast in a specimen which may be hard to see by bright field. (Talk recorded in July 2012)
Nico Stuurman describes different light sources for microscopy and shows a few examples of light sources that are currently used in microscopes. (Talk recorded in April 2012)
Harald Hess and Eric Betzig talk about the work that led to the development of the first super-high-resolution microscope for PALM microscopy. (Talk recorded in September 2010)
Nico Stuurman describes how fluorescence microscopy is a very powerful tool in Cell Biology research. (Talk recorded in June 2009)
Eva Nogales describes the principles and capabilities of transmission electron microscopy. (Talk recorded in June 2009)
Xiaowei Zhuang discusses how super-resolution microscopy allows scientists to obtain images with much better resolution and to study cell dynamics in greater detail. (Talk recorded in July 2011)